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The development of 3D culture models and organ-on-chip platforms brought a degree of complexity to classic in vitro for more physiological models of organs including the BBB

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After the reaction was complete, the solvent was distilled off under reduced pressure, and the residue was redissolved with ethyl acetate, extracted with 1M hydrochloric acid solution (50mL2), saturated sodium bicarbonate solution (50mL2), washed with saturated brine (75mL), The organic layer was collected and dried over anhydrous sodium sulfate

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